Laser Capture Microdissection and Two-Dimensional Polyacrylamide Gel Electrophoresis

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives.

The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms. While advances in mass spectrometry have been critical, developments made in two-dimensional PAGE (2D-PAGE) have also played a major role in enabling proteomic...

Two-Dimensional Polyacrylamide Gel Electrophoresis – A Practical Perspective

Twodimensional electrophoresis, abbreviated as 2-DE, is one of the most powerful and common tools for separation and fractionation of complex protein mixture extracted from tissues, cells, and other biological specimens. It is an orthogonal technique that allows separation of thousands of proteins in one gel and in a two tandem electrophoretic steps where a major proportion of proteins can be r...

Ongoing development of two-dimensional polyacrylamide gel electrophoresis data standards.

We present an approach toward standardizing two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) data in support of developing a globally relevant proteomics consensus in order to provide more efficient database querying and data comparisons through the establishment of the necessary definitions and interdisciplinary reference fields for both the 2-D PAGE community, particularly in the...

Polyacrylamide Gel Electrophoresis

Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...

Two-dimensional Gel Electrophoresis